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confocal microscope zeiss lsm 800  (Carl Zeiss)


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    Structured Review

    Carl Zeiss confocal microscope zeiss lsm 800
    Confocal Microscope Zeiss Lsm 800, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lsm+800/800+confocal+lsm+microscope/pmc12094565-112-10-12
    Average 90 stars, based on 1 article reviews
    confocal microscope zeiss lsm 800 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Incubation:

    Article Title: Daidzein alleviates skin fibrosis by suppressing TGF-β1 signaling pathway via targeting PKM2.
    Article Snippet: Cell proliferation was observed with the BeyoClickTM EdU-555 Imaging Kit (Beyotime Biotechnology, China). .. After 48 h treatment with different concentrations of Dai, an equal EDU was added for 2 h. Then, cells were fixed in 4% polymerization paraformaldehyde (PFA), and incubated with a Click Additive Solution for 30 min. Use LSM 800 (Zeiss, Germany) to focus on the microscope to detect immunofluorescence signals. ..

    Microscopy:

    Article Title: Daidzein alleviates skin fibrosis by suppressing TGF-β1 signaling pathway via targeting PKM2.
    Article Snippet: Cell proliferation was observed with the BeyoClickTM EdU-555 Imaging Kit (Beyotime Biotechnology, China). .. After 48 h treatment with different concentrations of Dai, an equal EDU was added for 2 h. Then, cells were fixed in 4% polymerization paraformaldehyde (PFA), and incubated with a Click Additive Solution for 30 min. Use LSM 800 (Zeiss, Germany) to focus on the microscope to detect immunofluorescence signals. ..

    Article Title: Daidzein alleviates skin fibrosis by suppressing TGF-β1 signaling pathway via targeting PKM2.
    Article Snippet: Cells were incubated with rabbit anti-α‐SMA (Affinity, AF1032, USA), anti-Collagen I (CST, 72026, USA) or anti-Phospho‐Smad3 (CST, 9520, USA) overnight at 4 °C, followed by FITC‐conjugated secondary antibody (Jackson, 115-095-003/111-025-003) and DAPI (Solarbio, S2110). .. Use LSM 800 (Zeiss, Germany) to focus on the microscope to detect immunofluorescence signals. ..

    Article Title: Cytoprotective Nanoencapsulation of Probiotic Cells within Fe 3+ -Phytic Acid Nanoshells.
    Article Snippet: Probiotic oral delivery is challenged by harsh gastrointestinal conditions and environmental stressors in the body.. This study presents the construction of durable nanoshells composed of an Fe3+phytic acid metal−organic complex around individual probiotic cells, providing exceptional cytoprotective properties, in single-cell nanoencapsulation.. Nanoencapsulated Levilactobacillus brevis maintains high viability under simulated gastric fluid and the antibiotic tetracycline.

    Immunofluorescence:

    Article Title: Daidzein alleviates skin fibrosis by suppressing TGF-β1 signaling pathway via targeting PKM2.
    Article Snippet: Cell proliferation was observed with the BeyoClickTM EdU-555 Imaging Kit (Beyotime Biotechnology, China). .. After 48 h treatment with different concentrations of Dai, an equal EDU was added for 2 h. Then, cells were fixed in 4% polymerization paraformaldehyde (PFA), and incubated with a Click Additive Solution for 30 min. Use LSM 800 (Zeiss, Germany) to focus on the microscope to detect immunofluorescence signals. ..

    Article Title: Daidzein alleviates skin fibrosis by suppressing TGF-β1 signaling pathway via targeting PKM2.
    Article Snippet: Cells were incubated with rabbit anti-α‐SMA (Affinity, AF1032, USA), anti-Collagen I (CST, 72026, USA) or anti-Phospho‐Smad3 (CST, 9520, USA) overnight at 4 °C, followed by FITC‐conjugated secondary antibody (Jackson, 115-095-003/111-025-003) and DAPI (Solarbio, S2110). .. Use LSM 800 (Zeiss, Germany) to focus on the microscope to detect immunofluorescence signals. ..

    Imaging:

    Article Title: Aging‐Associated Vacuolation of Multi‐Ciliated Cells in the Distal Mouse Oviduct Reflects Unique Cell Identity and Luminal Microenvironment
    Article Snippet: After six 30‐min‐long PBSMT washes, oviducts were transferred successively to methanol:PBT (1:1, PBT: 1× PBS and 1% Triton X‐100), 100% methanol, 3% H 2 O 2 (BioShop Canada Inc., HYP001.1), and 100% methanol prior to benzyl alcohol/benzyl benzoate (BABB) clearing. .. BABB‐cleared samples were placed on a #1.5 coverslip (Fisher Scientific, 12‐545F) with 10–15 μL BABB prior to imaging using the 10× objective (0.30 numerical aperture/N.A) on LSM 800 or 710 (Zeiss). ..

    Article Title: Cytoprotective Nanoencapsulation of Probiotic Cells within Fe 3+ -Phytic Acid Nanoshells.
    Article Snippet: Probiotic oral delivery is challenged by harsh gastrointestinal conditions and environmental stressors in the body.. This study presents the construction of durable nanoshells composed of an Fe3+phytic acid metal−organic complex around individual probiotic cells, providing exceptional cytoprotective properties, in single-cell nanoencapsulation.. Nanoencapsulated Levilactobacillus brevis maintains high viability under simulated gastric fluid and the antibiotic tetracycline.

    Article Title: Plasmid pPNptGreen Expression of Green Fluorescent Protein in Pseudomonas chlororaphis Strain S1Bt23 Abrogates Biocontrol Activity Against Pythium ultimum.
    Article Snippet: .. Intracellular and extracellular extracts from wild type S1Bt23 displayed potent antagonistic activity (Figure 4B; red disc) against Pythium ultimum, whereas extracts FIGURE 3 | Colonisation of 4- day old canola seedling roots by Pseudomonas chlororaphis S1Bt23- GFP observed and imaged with an LSM 800 (Carl Zeiss Imaging) using a Fluar 20X objective. ..

    other:

    Article Title: Advancing the Identification of Bioactive Molecules and the Construction of a Synergistic Drug Delivery System in Combating Lung Injury.
    Article Snippet: The authors also thank Xiaonan Ma for providing technical assistance of Carl Zeiss LSM 800 on the Public Experimental Platform of China Pharmaceutical University.

    Electron Microscopy:

    Article Title: Cytoprotective Nanoencapsulation of Probiotic Cells within Fe 3+ -Phytic Acid Nanoshells.
    Article Snippet: Probiotic oral delivery is challenged by harsh gastrointestinal conditions and environmental stressors in the body.. This study presents the construction of durable nanoshells composed of an Fe3+phytic acid metal−organic complex around individual probiotic cells, providing exceptional cytoprotective properties, in single-cell nanoencapsulation.. Nanoencapsulated Levilactobacillus brevis maintains high viability under simulated gastric fluid and the antibiotic tetracycline.

    Confocal Laser Scanning Microscopy:

    Article Title: Cytoprotective Nanoencapsulation of Probiotic Cells within Fe 3+ -Phytic Acid Nanoshells.
    Article Snippet: Probiotic oral delivery is challenged by harsh gastrointestinal conditions and environmental stressors in the body.. This study presents the construction of durable nanoshells composed of an Fe3+phytic acid metal−organic complex around individual probiotic cells, providing exceptional cytoprotective properties, in single-cell nanoencapsulation.. Nanoencapsulated Levilactobacillus brevis maintains high viability under simulated gastric fluid and the antibiotic tetracycline.

    Activity Assay:

    Article Title: Plasmid pPNptGreen Expression of Green Fluorescent Protein in Pseudomonas chlororaphis Strain S1Bt23 Abrogates Biocontrol Activity Against Pythium ultimum.
    Article Snippet: .. Intracellular and extracellular extracts from wild type S1Bt23 displayed potent antagonistic activity (Figure 4B; red disc) against Pythium ultimum, whereas extracts FIGURE 3 | Colonisation of 4- day old canola seedling roots by Pseudomonas chlororaphis S1Bt23- GFP observed and imaged with an LSM 800 (Carl Zeiss Imaging) using a Fluar 20X objective. ..

    Fluorescence In Situ Hybridization:

    Article Title: The transcription factor Traffic jam orchestrates the somatic piRNA pathway in Drosophila ovaries.
    Article Snippet: .. FISH and IF experiments were imaged using Zeiss LSM 800 or LSM 980 Airyscan confocal with a 20x and 403 objectives and analyzed using OMERO figure software. ..

    Software:

    Article Title: The transcription factor Traffic jam orchestrates the somatic piRNA pathway in Drosophila ovaries.
    Article Snippet: .. FISH and IF experiments were imaged using Zeiss LSM 800 or LSM 980 Airyscan confocal with a 20x and 403 objectives and analyzed using OMERO figure software. ..



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    Carl Zeiss laser confocal microscopy zeiss lsm 800
    RhCASPL1D1 interacts with RhPIP2s. A) Split-ubiquitin yeast two-hybrid assay. RhCASPL1D1 was fused to the N terminus of ubiquitin (RhCASPL1D1-Cub), and RhPIP2;1, RhPIP2;2 as well as RhPIP2;7 were fused to the C terminus of mutated ubiquitin (NubG-RhPIP2;1/RhPIP2;2/RhPIP2;7). The TSU2-APP/NubG-Fe65 and RhCASPL1D1-Cub/NubG combinations were used as controls. The transformed yeasts were plated on Trp-Leu-His-Ade-lacking synthetic defined medium to test protein interactions. B) Interaction of RhCASPL1D1 and RhPIP2s in bimolecular fluorescence complementation (BiFC) assay. RhCASPL1D1-cYFP and RhPIP2;1/RhPIP2;2/RhPIP2;7-nYFP constructs were co-infiltrated in N. benthamiana leaves. PM-mCherry was used as a plasmalemma marker. The cYFP/nYFP combination was used as a negative control. YFP fluorescence was visualized with confocal <t>microscopy</t> after 3-day infiltration. Scale bars, 50 μm. C) RhCASPL1D1-RhPIP2;7 interaction detected by co-IP. The combinations of RhCASPL1D1-FLAG and RhPIP2;7-MYC constructions were co-transformed into N. benthamiana leaves with negative control, GFP-FLAG/RhPIP2;7-MYC combination. Immunoprecipitation was conducted by incubation of leaf total proteins and FLAG beads. The input and co-immunoprecipitated proteins were analyzed using anti-FLAG and anti-MYC antibodies. D) RhCASPL1D1 co-localizes with RhPIP2;7 in N. benthamiana epidermal cells. RhCASPL1D1-GFP and RhPIP2;7-mCherry were co-expressed in N. benthamiana leaves. GFP + RhPIP2;7-mCherry, RhCASPL1D1-GFP + mCherry were regarded as negative control, respectively. Fluorescence was visualized via confocal microscopy 3 days after infiltration. The fluorescence intensity profiles along the white arrows (in the merge micrographs) were analyzed by ImageJ. Pearson correlation coefficients (Rp) for co-localization were calculated from the fluorescence signal in the white dashed boxes using ImageJ. Scale bars, 50 μm.
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    Image Search Results


    RhCASPL1D1 interacts with RhPIP2s. A) Split-ubiquitin yeast two-hybrid assay. RhCASPL1D1 was fused to the N terminus of ubiquitin (RhCASPL1D1-Cub), and RhPIP2;1, RhPIP2;2 as well as RhPIP2;7 were fused to the C terminus of mutated ubiquitin (NubG-RhPIP2;1/RhPIP2;2/RhPIP2;7). The TSU2-APP/NubG-Fe65 and RhCASPL1D1-Cub/NubG combinations were used as controls. The transformed yeasts were plated on Trp-Leu-His-Ade-lacking synthetic defined medium to test protein interactions. B) Interaction of RhCASPL1D1 and RhPIP2s in bimolecular fluorescence complementation (BiFC) assay. RhCASPL1D1-cYFP and RhPIP2;1/RhPIP2;2/RhPIP2;7-nYFP constructs were co-infiltrated in N. benthamiana leaves. PM-mCherry was used as a plasmalemma marker. The cYFP/nYFP combination was used as a negative control. YFP fluorescence was visualized with confocal microscopy after 3-day infiltration. Scale bars, 50 μm. C) RhCASPL1D1-RhPIP2;7 interaction detected by co-IP. The combinations of RhCASPL1D1-FLAG and RhPIP2;7-MYC constructions were co-transformed into N. benthamiana leaves with negative control, GFP-FLAG/RhPIP2;7-MYC combination. Immunoprecipitation was conducted by incubation of leaf total proteins and FLAG beads. The input and co-immunoprecipitated proteins were analyzed using anti-FLAG and anti-MYC antibodies. D) RhCASPL1D1 co-localizes with RhPIP2;7 in N. benthamiana epidermal cells. RhCASPL1D1-GFP and RhPIP2;7-mCherry were co-expressed in N. benthamiana leaves. GFP + RhPIP2;7-mCherry, RhCASPL1D1-GFP + mCherry were regarded as negative control, respectively. Fluorescence was visualized via confocal microscopy 3 days after infiltration. The fluorescence intensity profiles along the white arrows (in the merge micrographs) were analyzed by ImageJ. Pearson correlation coefficients (Rp) for co-localization were calculated from the fluorescence signal in the white dashed boxes using ImageJ. Scale bars, 50 μm.

    Journal: Horticulture Research

    Article Title: Scaffold protein RhCASPL1D1 stabilizes RhPIP2 aquaporins and promotes flower recovery after dehydration in rose ( Rosa hybrida )

    doi: 10.1093/hr/uhaf119

    Figure Lengend Snippet: RhCASPL1D1 interacts with RhPIP2s. A) Split-ubiquitin yeast two-hybrid assay. RhCASPL1D1 was fused to the N terminus of ubiquitin (RhCASPL1D1-Cub), and RhPIP2;1, RhPIP2;2 as well as RhPIP2;7 were fused to the C terminus of mutated ubiquitin (NubG-RhPIP2;1/RhPIP2;2/RhPIP2;7). The TSU2-APP/NubG-Fe65 and RhCASPL1D1-Cub/NubG combinations were used as controls. The transformed yeasts were plated on Trp-Leu-His-Ade-lacking synthetic defined medium to test protein interactions. B) Interaction of RhCASPL1D1 and RhPIP2s in bimolecular fluorescence complementation (BiFC) assay. RhCASPL1D1-cYFP and RhPIP2;1/RhPIP2;2/RhPIP2;7-nYFP constructs were co-infiltrated in N. benthamiana leaves. PM-mCherry was used as a plasmalemma marker. The cYFP/nYFP combination was used as a negative control. YFP fluorescence was visualized with confocal microscopy after 3-day infiltration. Scale bars, 50 μm. C) RhCASPL1D1-RhPIP2;7 interaction detected by co-IP. The combinations of RhCASPL1D1-FLAG and RhPIP2;7-MYC constructions were co-transformed into N. benthamiana leaves with negative control, GFP-FLAG/RhPIP2;7-MYC combination. Immunoprecipitation was conducted by incubation of leaf total proteins and FLAG beads. The input and co-immunoprecipitated proteins were analyzed using anti-FLAG and anti-MYC antibodies. D) RhCASPL1D1 co-localizes with RhPIP2;7 in N. benthamiana epidermal cells. RhCASPL1D1-GFP and RhPIP2;7-mCherry were co-expressed in N. benthamiana leaves. GFP + RhPIP2;7-mCherry, RhCASPL1D1-GFP + mCherry were regarded as negative control, respectively. Fluorescence was visualized via confocal microscopy 3 days after infiltration. The fluorescence intensity profiles along the white arrows (in the merge micrographs) were analyzed by ImageJ. Pearson correlation coefficients (Rp) for co-localization were calculated from the fluorescence signal in the white dashed boxes using ImageJ. Scale bars, 50 μm.

    Article Snippet: Two days after infiltration, the co-localization of GFP and mCherry was observed with a laser confocal microscopy (Zeiss LSM 800).

    Techniques: Ubiquitin Proteomics, Y2H Assay, Transformation Assay, Bimolecular Fluorescence Complementation Assay, Construct, Marker, Negative Control, Fluorescence, Confocal Microscopy, Co-Immunoprecipitation Assay, Immunoprecipitation, Incubation